hDLL3/KLN205
CBPG0126
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| I. Background | |
| DLL3 基因別名 SCDO1,其編碼的蛋白 DLL3 是一種跨膜蛋白,是 Notch 受體配體 Delta/Serrate/lag2(DSL)組的成員,在胚層的分離中,影響著(zhù)體細胞邊界的形成。 DLL3 基因突變會(huì )導致嚴重的體節發(fā)生異常和脊椎肋骨發(fā)育不全。DLL3 是一種高度腫瘤選 擇性的細胞表面靶點(diǎn),在正常肺癌組織及癌旁組織中不表達。DLL3 在多種腫瘤中表達上 調,在小細胞肺癌、乳腺癌、垂體瘤、急性髓系白血病中有促癌作用,但在肝癌 、神經(jīng)膠 質(zhì)瘤和惡性膠質(zhì)瘤中卻發(fā)揮了抑癌作用。 | |
| II. Introduction | |
| Host Cell: | KLN205 |
| Expressed gene: | hDLL3 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM+ 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +2μg/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mus musculus, mouse |
| Tissue: | Lung |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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Figure 1. Recombinant hDLL3/KLN205 stably expressing hDLL3. |
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