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c-Kit D816V/BaF3

CBP73206

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I. Introduction
Cell Line Name: c-Kit D816V/BaF3
Host Cell: Ba/F3
Stability: 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application: Anti-proliferation assay and PD assay
Freeze Medium: 90% FBS+10% DMSO
Complete Culture Medium: RPMI-1640+10%FBS
Mycoplasma Status: Negative
 
II. Background

Human cKit with D816V substitution, GenBank Accession No. NM_000222, a.a. 544-end with N-terminal His- tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=53 kDa.

 
III. Representative Data

1.WB of  c-Kit D816V/BaF3

2. Sanger of c-Kit D816V/BaF3

3. Anti-proliferation assay

Figure 3. Anti-proliferation assay of three reference compounds on the c-Kit D816V/BaF3.

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