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首頁(yè) /科研細胞 /人源細胞系 /子宮 /AN3-CA(人子宮內膜腺癌細胞)

AN3-CA(人子宮內膜腺癌細胞)

CBP60663

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I. General information 
Synonyms: AN3-CA
Species: Homo sapiens, human
Tissue: uterus; endometrium
Disease: adenocarcinoma
Gender: Caucasian,female,55years
Morphology: epithelial
Growth Mode: adherent
Doubling Time: 32 hrs
DNA Profile: Amelogenin: X
CSF1PO: 12,14,15
D13S317: 12,14
D16S539: 10,14
D5S818: 11,14
D7S820: 7,10,7.1
THO1: 10,9.3
TPOX: 8,10
vWA: 14,20
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Culture Medium:

MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)

AN3-CA完全培養基,# CBP60663M
We strongly suggest to purchase the complete medium from us.

Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: Culture medium, 95%; DMSO, 5%
Tumor Formation: Yes, in nude mice,Yes, in the cheek pouch of cortisone treated hamsters
Comments: The cells exhibit ion transport activities consistent with cystic fibrosis and express the product of the CF gene (cystic fibrosis transmembrane regulator, CFTR).CFPAC-1 cells show no effect of cAMP agonists, adenyl cyclase stimulators or phosphodiesterase inhibitors on Cl- flux, but do respond to Ca++ ionophores with increase Cl- efflux.
The cells have the most common form of the CF mutation, deletion of three nucleotides resulting in the absence of phenylalanine at position 508.CFPAC-1 cells have epithelial morphology and polarization with apical microvilli, tight junctions and gap junctions.
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